Mammalian Erythrocyte Micronucleus Test

The mammalian in vivo micronucleus test is used for the detection of damage induced by the test substance to the chromosomes or the mitotic apparatus of erythroblasts, by analysis of erythrocytes as sampled in bone marrow and/or peripheral blood cells of animals, usually rodents (mice or rats).

Test ModelMice, Rats
Assay ControlsVehicle Control: Appropriate vehicle control
Positive Control: Cyclophosphamide monohydrate
Exposure Time24 and 48 hours. Integrated with repeat dose toxicology studies
Test Item QuantityTop dose concentration – Quantity may vary 2000 mg/kg – 13.2 grams
End PointBone marrow or Blood Micronucleus (4000 Polychromatic erythrocytes/Reticulocytes)
Data DeliveryPercentage of micronuclei compared with vehicle control
Timeline11 – 12 Weeks

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