In Vitro Micronucleus Test

The in vitro micronucleus test is a genotoxicity test for the detection
of micronuclei in the cytoplasm of interphase cells.

Test ModelChinese Hamster Ovary (CHO), Human peripheral blood lymphocytes
Assay ControlsNegative Control – Aqueous and Organic solvents
Positive Control – Methyl Methane Sulphonate/Ethyl Methane Sulphonate, Cyclophosphamide
monohydrate and Vinblastine
Exposure TimeShort Duration – (With and Without Metabolic Activation, 3 – 6 hrs)
Prolonged duration treatment: (Without Metabolic Activation, 20 – 21 hrs)
Test Item Concentration10 mM, 2 mg/mL or 2 μl/mL
End PointMicronucleus evaluation in 2000 binucleate cells
Data DeliveryPercentage of micronuclei and Replication Index
Timeline10 – 12 Weeks

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