In Vitro 3T3 NRU Phototoxicity Test

The in vitro 3T3 Neutral Red Uptake (NRU) phototoxicity test is used to identify the phototoxic potential of a test chemical activated by exposure to light. The test evaluates photo-cytotoxicity by the relative reduction in viability of cells exposed to the test chemical in the presence versus absence of light

Test ModelBalb/c 3T3 cells
ReplicatesSix per group (Preliminary and Confirmatory study)
Assay ControlsNegative Control – Aqueous or organic solvents
Positive Control – Chlorpromazine (CPZ)
Eight different Test Item concentrations
Exposure Time5 J/CM2 for 50 minutes
Test Item Quantity1.0 gram
End PointThe ability of viable cells to incorporate and bind neutral red (NR) in the presence and absence of light
Data DeliveryIC50 Calculation and Photo-Irritation Factor
Timeline3 – 4 Weeks

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