In Vitro 3T3 NRU Cytotoxicity Test

The Neutral red uptake (NRU) cytotoxicity test is based on the ability of viable cells to incorporate and bind neutral red (NR).

Test ModelBalb/c 3T3 cells
ReplicatesSix per group (Preliminary and Confirmatory study)
Assay ControlsNegative Control – Aqueous or Organic Solvents
Positive Control – Sodium Lauryl Sulfate
Eight different Test Item concentrations
Exposure Time44 – 48 hours
Test Item Quantity1.0 gram
End PointThe ability of viable cells to incorporate and bind neutral red (NR)
Data Delivery% of viability of cells
Timeline3 – 4 Weeks

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